Tag Archives: Build Options

Glycosylating Erythropoietin Using 1BUY.pdb

Overview In this exercise, we will: Download a PDB structure of a mutated protein. In its native state, the protein is glycosylated, and it does not perform its functions properly without at least some of the glycosylation. Use the text editor and the utilities at GLYCAM-Web to change the mutated residues back to the natural […]

Should I solvate and/or add counter ions?

This question references the Options available when¬†using the Glycoprotein Builder or any of the various interfaces to the Carbohydrate Builder (e.g., GAG Builder, Build-by-URL, Interactive Builder). In general, if you do not know for certain that you need to solvate or add counter ions, then you do not need to. ¬†These options are typically only […]